Abstract
5-Hydroxymethylcytosine (5-hmC) is a newly discovered DNA modification in mammalian genomes. This protocol is to be a highly efficient and selective chemical approach to label and capture 5-hmC, taking advantage of a bacteriophage enzyme that adds a glucose moiety to 5-hmC specifically, which could in turn be used for high-throughput mapping via next-generation sequencing.
Keywords: 5-hydroxymethylcytocine, Capture, Sequencing
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
The protocol described here is adapted from one reported previously (Song et al., 2011).
References
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