Abstract
Primer extension analysis is a useful method to determine the transcription start point or a processing site on an RNA molecule. It can also allow a quantitative measurement of an RNA species.
Keywords: 5' end mapping, Reverse Transcription, Primer extension
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This laboratory protocol is a free adaption of various published and unpublished protocols and has evolved over time. We acknowledge the support by funds from the CNRS (UPR 9073) and Univ Paris Diderot, Sorbonne Paris Cite.
References
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