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Photometric Assays for Chloroplast Movement Responses to Blue Light   

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Original research article

A brief version of this protocol appeared in:
Journal of Experimental Botany
Sep 2016

Abstract

Assessment of chloroplast movements by measuring changes in leaf transmittance is discussed, with special reference to the conditions necessary for reliable estimation of blue light–activated chloroplast responses.

Keywords: Arabidopsis thaliana, Blue light, Chloroplast movements, Leaf transmittance, Photometric method, Phototropins

Background

Following the discovery of phototropins, chloroplast movements activated with blue light absorbed by these photoreceptors began to arouse much interest. Quantitative assessment of chloroplast redistribution in a multilayer leaf relies mainly on measuring transmittance changes of the tissue that are a consequence of this redistribution. Various devices have been used for that purpose, including a recently adapted commercial microplate reader (Wada and Kong, 2011; Johansson and Zeidler, 2016), particularly suited for screening large number of samples. However, samples must be properly pretreated and characterized to make their comparison reliable. This aspect has not been referred to in many papers making use of the transmittance technique. Hence, we found it important to discuss these issues in the current protocol.

Copyright: © 2017 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Gabryś, H., Banaś, A. K., Hermanowicz, P., Krzeszowiec, W., Leśniewski, S., Łabuz, J. and Sztatelman, O. (2017). Photometric Assays for Chloroplast Movement Responses to Blue Light. Bio-protocol 7(11): e2310. DOI: 10.21769/BioProtoc.2310.
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