Published: Vol 7, Iss 10, May 20, 2017 DOI: 10.21769/BioProtoc.2283 Views: 9627
Reviewed by: Xi FengManuel SarmientoAnonymous reviewer(s)
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Abstract
This protocol describes a simple locomotor assay in Drosophila melanogaster. In brief, the locomotor of each single fly in the culture dish is recorded by a web camera. The moving time, walking length, speed and the locomotor trails of the single fly could be quantitatively analyzed.
Keywords: LocomotorBackground
This protocol was implemented in the previously published study (Liu et al., 2016). In that study, this assay was combined with the optogenetic system by simply providing the proper excitation light.
Materials and Reagents
Equipment
Software
Procedure
Data analysis
Acknowledgments
We would like to thank Jingwu Hou for assistance with experimental setup. This protocol was designed by Q. L. and was implemented in the previously published study (Liu et al., 2016). This study was supported by the ‘Strategic Priority Research Program’ of the CAS (XDB02040004), by grants from the 973 Program (2011CBA00400), as well as by the National Science Foundation of China (91232000, 91132709, 31130027, and 31070956). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
References
Article Information
Copyright
Liu et al. This article is distributed under the terms of the Creative Commons Attribution License (CC BY 4.0).
How to cite
Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
Category
Neuroscience > Behavioral neuroscience > Learning and memory
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