Abstract
1-MCP (1-methylcyclopropene) is a simple synthetic hydrocarbon molecule that interacts with the ethylene receptor and inhibits the response of fruit or plant to ethylene. 1-MCP has opened new opportunities in handling harvested crops and serves as a powerful tool to learn about plant response to ethylene (Watkins and Miller, 2006). 1-MCP is manufactured by Agrofresh and known by its commercial name SmartfreshSM.
Keywords: Ripening inhibition, Ethylene, Fruit, Postharvest, Storage
Background
Application of 1-MCP can serve as a powerful tool to examine the response of the plant to ethylene. The commercial production of 1-MCP by Agrofresh allows simple practice of this treatment, especially postharvest. The application of 1-MCP to plants, fruit or vegetable was mentioned in many articles but was not described in detail. This was the motivation to provide the following protocol. In this protocol tomatoes served as candidates for the treatment.
Materials and Reagents
Equipment
Procedure
Notes
Acknowledgments
The protocol was adapted from Gamrasni et al. (2010), and was similarly applied in Breitel et al. (2016). We would like to thank RIMI and Agrofresh Inc. for the supply of 1-MCP.
References
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.
Hi,You should do similar to the protocol,However apply 100 mL of 1-MCP gas instead of 30 mL as mentioned in procedure section 2b if you need 1 ppm.Good Luck!Dan
Dear Dirier!How are you!!! Iy=t is Danny from Israel.If you have any problems with that please let me know.I do this protocol very often with no problems.RegardsDannydanny.gamrasni@gmail.com
o.k.Whenever you need it and there might be any questions you are welcome to ask me.Regards,Dan