Pathogenicity Assay of Penicillium expansum on Apple Fruits   

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Original research article

A brief version of this protocol appeared in:
Molecular Plant Microbe Interactions
Jun 2015


Penicillium expansum, a widespread filamentous fungus, is a major causative agent of fruit decay and leads to huge economic losses during postharvest storage and shipping. Furthermore, it produces mycotoxin on the infected fruits that may cause harmful effects to human health. This pathogenicity assay involves a stab inoculation procedure of P. expansum on apple fruit, an important experimental technique to study fungal pathogenesis. This assay can be applied to analyze the virulence of postharvest pathogen on other fruits such as orange, pear and kiwifruit.

Keywords: Penicillium expansum, Apple fruit, Stab inoculation, Pathogenicity assay


Penicillium expansum is a destructive postharvest pathogen that causes decay in many popular fruits, such as apple and pear, during postharvest handling and storage. It causes significant socioeconomic impacts and has implications for international trade. The pathogen can also lead to serious health problems in human since it produces toxic secondary metabolites, including patulin, citrinin, and chaetoglobosins (Andersen et al., 2004). Control of decay caused by P. expansum has become important for ensuring the quality and safety of various fruits.

Conidia of P. expansum typically enter through wounds, which is necessary to provide sites for initiation of the pathogen development (Spotts et al., 1998). Pathogenicity of P. expansum on fruits is usually tested by needle-stab inoculation, which is also used for pathogenicity assays of Botrytis cinerea vs. tomato, Monilinia fructicola vs. peach, Colletotrichum gloeosporioides vs. mango, etc. (Liu et al., 2012; Shi et al., 2012; Zhang et al., 2014). Here, we described a protocol to assess pathogenicity of P. expansum on apple fruits based on stab inoculation method.

Copyright: © 2017 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Chen, Y., Li, B., Zhang, Z. and Tian, S. (2017). Pathogenicity Assay of Penicillium expansum on Apple Fruits. Bio-protocol 7(9): e2264. DOI: 10.21769/BioProtoc.2264.

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