Hi,
Nice protocol. I have a few questions.
1) our spectrophotometer does not have a 540nm filter. We do have 535 and the tech seemed to think that this was "close enough". any thoughts on that?
2) what is your procedure for creating the glycerol standard titrations to generate the regression line from the data?
3) is there a way to add steps to obtain a protein fraction from this protocol?
4) we are planning to use the protocol on both muscle and triglyceride (which the literature says is fine). have you had success with both?
5) we may not have 100mg of tissue for all samples. if we have 50mg, for example, must we adjust the volume of all the reagents or does this not matter b/c the calculations are based on total weight of tissue?
Thanks a lot!
3/20/2013 4:23:28 PM Reply