Dear Dr. Muschel,
Thank you very much for providing such detailed protocol!
However, I've noticed several key typos regarding the recipes of the "washing buffer" and "resuspension buffer":
-Washing buffer: based on the final EGTA concentration being 1mM, the ratio of the 100mM EGTA to resuspension buffer should have been 1ml to 100ml, instead of "1ml to 2ml"
-Resuspension buffer: since the NaHCO3's final concentration should be around 12mM (and your 2004 Cancer Res paper did accurately described that), the volume of 7.5% NaHCO3 should have been 2ml instead of "100ml"
Again, really appreciate your protocol!
1/15/2019 9:53:47 AM Reply