Abstract
Estimation of circulating immune complex in tuberculosis patients has shown better insight to the infection. Isolating circulating immune complex helps quantifying both antigen and antibody in the serum. It’s a simple procedure to improve the sensitivity and specificity in serodiagnosis of tuberculosis. This protocol may be modified to detect antigen/antibody in other infectious diseases.
Keywords: Circulating Immune complex, Serodiagnosis, Tuberculosis, TB diagnosis, Antigen and antibody detection
Materials and Reagents
Equipment
Procedure
Notes
The samples to be assayed in a plate were randomly allocated to different wells within the plate and were also coded to conceal the identity of the specimens. In each of the experimental replicates of a positive reference serum (serum which has a strong positive response with high concentration of antibody from a smear microscopy confirmed tuberculous patient) was included. The mean value of the positive reference serum in independent experiments was taken as a constant reference value to assess plate-to-plate and day-to-day variations. Each plate had blank wells which represents the wells without antibody binding.
Recipes
Acknowledgments
This protocol was adopted from previous work published by Raja et al. (1995).
References
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