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Determination of Intra- and Extracellular Glucose in Mycelium of Fusarium oxysporum   

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Original research article

A brief version of this protocol appeared in:
Molecular Plant Microbe Interactions
Jan 2015

Abstract

To study alterations in the metabolism and/or in the transport of glucose during Fusarium oxysporum vegetative growth, we determined intracellular glucose levels in different fungal strains, as well as the amount of glucose remaining in the supernatants after growth in synthetic medium (SM) supplemented with either 0.05 or 2.5% glucose. We used the Glucose (GO) Assay Kit (Sigma-Aldrich) following the instructions of the manufacturer with some modifications. The protocol described here can be applied to other filamentous fungi.

Keywords: Glucose determination, Fusarium, Glucose utilization

Copyright: © 2016 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Ruiz-Roldan, C. and Roncero, M. I. (2016). Determination of Intra- and Extracellular Glucose in Mycelium of Fusarium oxysporum. Bio-protocol 6(14): e1868. DOI: 10.21769/BioProtoc.1868.
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