Abstract
To study alterations in the metabolism and/or in the transport of glucose during Fusarium oxysporum vegetative growth, we determined intracellular glucose levels in different fungal strains, as well as the amount of glucose remaining in the supernatants after growth in synthetic medium (SM) supplemented with either 0.05 or 2.5% glucose. We used the Glucose (GO) Assay Kit (Sigma-Aldrich) following the instructions of the manufacturer with some modifications. The protocol described here can be applied to other filamentous fungi.
Keywords: Glucose determination, Fusarium, Glucose utilization
Materials and Reagents
Equipment
Procedure
Representative data
Recipes
Acknowledgments
This research was supported by Junta de Andalucia (Proyecto de Excelencia CVI-7319) and the Spanish Ministerio de Economia y Competitividad (grant BIO2013-47870 and the Ramon y Cajal Program). This protocol was adapted from Ruiz-Roldan et al., 2015.
References
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