T Cell Transfer Model of Colitis   

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Original research article

A brief version of this protocol appeared in:
Nature Communications
May 2015


Inflammatory bowel disease (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC) is mainly caused by disordered immune regulation and dysregulated intestinal microbiota. Here we present the T cell transfer model which has extensively used in many studies to identify the regulatory T cell function in gut inflammation. Naïve T cells only or together with Treg cells isolated from different donors were transferred into immunodeficient Rag1-/- mice and the disease progression was assessed by the loss of body weight and the scoring analysis. This model provides a reliable work system for the study of gut inflammation.

Keywords: Induced colitis, Mouse model, T cells, Gut inflammation, Rag1-/-

Copyright: © 2016 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Pan, W., Zhu, S., Dai, D., Tang, Y., Yao, Y. and Shen, N. (2016). T Cell Transfer Model of Colitis. Bio-protocol 6(13): e1862. DOI: 10.21769/BioProtoc.1862.

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