Abstract
Detection of natural hybrids is of great significance for plant taxonomy, reproductive biology, and population genetic studies. Compared with methods depending on morphological characters, molecular markers provide reliable and much more accurate results. This protocol describes approaches employing microsatellite (SSR) markers to identify inter-specific hybrids in Mussaenda (Rubiaceae).
Keywords: Gene flow, Natural hybridization, SSR
Materials and Reagents
Equipment
Software
Procedure
Representative data
A representative example of data can be found in our published work (Luo et al., 2015). Figure 1 shows genotype class analysis of all sampled individuals based on the programs NewHybrids and STRUCTURE using SSR data. Figure 1. Genotype class analysis of all sampled individuals based on the programs NewHybrids (A) and STRUCTURE (B) using SSR data. A. NewHybrids calculated posterior probability that falls into each of six genotype categories: P1 (Parent 1), P2 (Parent 2), F1 (F1 hybrids), F2 (F2 hybrids), BC 1 (backcross generation to Parent 1), BC 2 (backcross generation to Parent 2). B. Structure of 81 accessions based on 13 SSR loci (K = 2). Each bar represents one individual, P1 = M. kwangtungensis, P2 = M. pubescens var. alba.
Recipes
Acknowledgments
This work was supported by the National Natural Science Foundation of China (31000109, 31370269, 31170184, U1202261) and the International Foundation for Science (AD/22476). This protocol was modified from our previous work, Luo, Z. L., Duan, T. T., Yuan, S., Chen, S., Bai, X. F., and Zhang, D. X. (2015) Reproductive isolation between sympatric sister species, Mussaenda kwangtungensis and M. pubescens var. alba. J Integr Plant Biol 57: 859-870.
References
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