Abstract
Plant parasitic nematodes parasitize roots and/or stems of various plants thereby inhibiting absorption of nutrients and moisture. In particular, root-knot nematodes (RKN) are a group of the most devastating pests. Various techniques, such as soil sterilization, cultivation of resistant crops, and chemical application, have been developed to control damage caused by RKN. Among these techniques, diminish by chemicals that induce or activate host defense to RKN is an attractive method because of its potential to reduce the environmental burden caused by crop protection. Sclareol, a diterpene, was identified as a chemical that induces resistance to RKN (Fujimoto et al., 2015). Here we provide a protocol for assessing the impact of sclareol on the penetration of RKNs into tomato and Arabidopsis roots and the direct nematicidal impact of the chemical on nematodes. This protocol can be used for other nematode resistance-inducing chemicals.
Keywords: Root-knot nematode, Nematicidal activity, Nematode penetration, Stain method
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, the Japanese Program for the Promotion of Basic and Applied Research for Innovation in Bio-oriented Industry (BRAIN), Japan, and a grant from Cross-ministerial Strategic Innovation Promotion Program, Japan.
References
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