Abstract
The opportunistic fungal human and plant pathogen Aspergillus terreus (A. terreus) can be isolated from sea water, soil or decaying organic matter such as rotting leaves and fruits. While growing on fruits A. terreus produces secondary metabolites such as terrein, which may ease its penetration into plant tissues. In addition, biological activities of terrein may support competition against other microorganisms. In summary, terrein is a small polyketide that reduces germination of seedlings, induces lesions on fruit surfaces but also shows moderate antifungal activity. With this manuscript we provide a fruit infection protocol with Aspergillus terreus with subsequent determination of terrein production rates on infected fruits using an HPLC-based quantification approach.
Keywords: Aspergillus terreus, Terrein, Secondary metabolite quantification, Ethyl acetate extraction, Banana
Materials and Reagents
Equipment
Procedure
Notes
Recipes
Acknowledgments
This protocol gives a detailed description of methods published previously in Gressler et al. (2015) and Zaehle et al. (2014). This work was financially supported by the German Science Foundation (DFG grant BR 2216/4-1) and internal funding from the Hans Knöll Institute, Jena (Germany).
References
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