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Zebrafish Embryo DNA Preparation
Published: Feb 20, 2012 DOI: 10.21769/BioProtoc.184 Views: 21485
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Abstract
This protocol explains how to extract DNA from a single zebrafish embryo. It does not require the use of expensive kits.
Background
Materials and Reagents
- Proteinase K (Roche Diagnostics, catalog number: 03115836001 )
- 1 M Tris (pH 8.3)
- NaCl
- KCl
- CaCl2·2H2O
- MgSO4·7H2O
- Sterile water
- 10%Tween 20 (EMD Biosciences, catalog number: 655207 )
- 10% NP40 (Merck KGaA, catalog number: 492018 )
- Embryo lysis buffer (see Recipes)
- 1x PCR buffer (see Recipes)
- E3 (see Recipes)
Equipment
- PCR Thermal cycler
- Centrifuges
- Incubator
- 96-well plate
Procedure
Copyright: © 2012 The Authors; exclusive licensee Bio-protocol LLC.
Category
Molecular Biology > DNA > DNA extraction
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