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Hi,I tried your protocol to extract gDNA from a single embryo...

Özge Çark
Özge Çark Jul 12, 2019
protocol Protocol: Zebrafish Embryo DNA Preparation
Hi,

I tried your protocol to extract gDNA from a single embryo for mutation screening. Although my primers are specific for the target region at 60C annealing temp in my other PCRs, when I use gDNA from this protocol, I obtained nonspecific bands. What could be happened in the r Read more Read less more less
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Özge Çark Answered Jul 16, 2019

CRTD

Thank you :) I will try to adjust annealing temp.
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ajay deepak verma Answered Jul 16, 2019

centre for cellular and molecular biology

Hi,
This happened with one of my primer pair too but worked by adjusting the annealing temperature. It might be due to high salts.
Good luck,
Ajay
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