Abstract
Transepithelial Electrical Resistance (TER) measurement is a reliable and efficient method to quantify the permeability of barrier forming cells such as epithelial cells. Measuring the permeability of the epithelial cells will help the researchers to investigate the barrier function of epithelium in various infectious and inflammatory diseases. Here we provide a real-time and impedance-based approach for measuring the permeability of epithelial cell monolayer using the Electrical Cell Substrate Impedance Sensing (ECIS®) instrumentation.
Keywords: ECIS, Barrire, Epithelium
Materials and Reagents
Equipment
Software
Procedure
Notes
Recipes
Acknowledgments
The protocol had been adapted from a previously published paper (Meng et al., 2015) and the Ztheta Manual provided by the Applied Biophysics, Inc. This work was supported in part by the NIH grants RO1 DA 12104, RO1 DA 022935, RO1 DA031202, K05DA033881, P50 DA 011806 and 1R01DA034582 to S. Roy.
References
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