Published: Vol 6, Iss 10, May 20, 2016 DOI: 10.21769/BioProtoc.1808 Views: 7959
Reviewed by: Maria SinetovaClaudia CatalanottiAnonymous reviewer(s)
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Abstract
Akinetes are spore-like resting (dormant) cells formed by strains of filamentous cyanobacteria for surviving long periods of unfavorable conditions. During deprivation for potassium, vegetative photosynthetic cells along the filaments of the cyanobacterium Aphanizomenon ovalisporum (A. ovalisporum) (strain ILC-164) differentiate into akinetes. Akinetes are larger than vegetative cell, have a thick wall, accumulate storage compounds (cyanophycine, glycogen, lipids) and excess of DNA (Sukenik et al., 2015; Sukenik et al., 2007; Maldener et al., 2014). Differences in structure and composition between akinetes and vegetative cells allow separation and isolation of akinetes. Akinetes isolated by the described protocol can be utilized for protein analysis, measurements of metabolic activities, fluorescence in situ hybridization (FISH) studies and more.
Materials and Reagents
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Acknowledgments
The protocol was modified from Razquin et al. (1996). The work was supported by research grants from the Israel Science Foundation (ISF grant No. 319/12), a Joint German-Israeli-Project (FKZ 02WT0985, WR803) from German Ministry of Research and Technology (BMBF) and Israel Ministry of Science and Technology (MOST). R. N. K-L was supported by Levy Eshkol Fellowship (No. 3-9476) Israel Ministry of Science and Technology (MOST).
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Article Information
Copyright
© 2016 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Sukenik, A., Kaplan-Levi, R. N., Viner-Mozzini, Y., Lupu, A. and Sela, D. (2016). Induction, Isolation and Counting of Akinetes in Aphanizomenon ovalisporum. Bio-protocol 6(10): e1808. DOI: 10.21769/BioProtoc.1808.
Category
Microbiology > Microbial cell biology > Cell isolation and culture
Microbiology > Microbial cell biology > Cell viability
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