Abstract
Burkholderia glumae (B. glumae) is becoming a serious threat in the major rice producing areas of the world. It was reported that Burkholderia spp., including B. glumae, are adapted to a wide range of ecological niches. Different bacterial strains show different levels of UV tolerance which may be due to the presence of different protection mechanisms. Previously we reported that pigment producing strains of B. glumae are more tolerant to UV radiation than non-pigmented strains. Here, we describe the protocol of UV tolerance assay for B. glumae in different exposure times. Using this protocol, we can calculate the survival rate of B. glumae strains, as well as other bacterial species, in exposure to UV radiation.
Materials and Reagents
Equipment
Procedure
Note: For handling bacterial cultures, aseptic techniques must be used and each step should be carried out in a sterile laminar flow hood when practicable. Day 1
Day 2
Figure 1. Strains of Burkholderia glumae grown on CPG agar plates. The upper-left and bottom plates: Pigment-producing strains of B. glumae. The upper-right plate: A pigment-deficient strain of B. glumae. Photo was taken after 48 h of incubation at 30 °C.
Day 4
Day 6
Recipes
Acknowledgments
This work was supported by the USDA NIFA (Hatch Project #: LAB93918 and LAB94203), the Louisiana State University Agricultural Center, and the Louisiana Board of Regents (the Research and Development Program: LEQSF(2008-11)-RD-A-02). This protocol was modified from our previous work published in PLoS ONE (Karki et al., 2012).
References
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