Published: Vol 6, Iss 5, Mar 5, 2016 DOI: 10.21769/BioProtoc.1750 Views: 11613
Reviewed by: Ivan ZanoniRamalingam BethunaickanBenoit Chassaing
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Abstract
Dendritic cells (DCs) play a critical role in mounting the T cell response against different infectious agents. Nature and intensity of the induced T cell responses are defined by activation status of DCs. It is generally accepted that IL-12, IL-4/IL-5 and IL-23 producing DCs induce TH1, TH2 and TH17 type of immune responses, respectively (Kumar et al., 2015). Besides cytokines, levels of co-stimulatory molecules on DCs also influence the response of T cells.
The activation status of DCs can be determined by examining DC culture supernatants for different cytokines and by analyzing expression of co-stimulatory molecules on these cells. However, these approaches provide indirect information about T cell activating potential of DCs. Analysis of T cell responses in a co-culture system is a more direct approach to examine T cell proliferating and polarizing efficacy of DCs.
A protocol to analyze the T cell proliferating and polarizing potential of DCs in an allogeneic mixed leukocyte reaction (allo-MLR) is described here.
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Copyright
© 2016 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Kumar, P. and Bhaskar, S. (2016). Analysis of T Cell Proliferating and Polarizing Potential of Murine Dendritic Cells in Allogeneic-mixed Leukocyte Reaction. Bio-protocol 6(5): e1750. DOI: 10.21769/BioProtoc.1750.
Category
Immunology > Immune cell function > Dendritic cell
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