Abstract
The detailed protocol is used to isolate different cell types from murine brain as glial cells, including microglia, using an enzymatic digestion that minimizes cellular mortality. A Percoll gradient (30% to 80%) separation allows a maximal recovery of isolated murine microglial cells prior to flow cytometry analysis.
Keywords: Flow Cytometry, Microglial cell, Mouse, Brain
Materials and Reagents
Equipment
Software
Procedure
Representative data
Figure 2. Example of results following an analysis of isolated microglial cells by flow cytometry. Dot plot showing a representative sample of isolated microglial cells analyzed by flow cytometry following a Live/Dead-Blue, CD45-PE-Cy5 and CD11b-AF700 immunofluorescent staining.
Recipes
Acknowledgments
This work was supported by grants from the Canadian Institutes for Health Research (CIHR) and the Multiple Sclerosis Scientific Research Foundation of Canada. This protocol was adapted from previous work of Martine Lessard and Antoine Lampron.
References
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