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Neurite Outgrowth Assay   

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Abstract

Neurite outgrowth in culture provides an easy way to determine the effects of a particular substrate or exogenous factor on neuron behavior. Dissociated neurons can be plated on a variety of substrates and the length of the longest neurite outgrowth can be compared. Here, we describe how to isolate and dissociate dorsal root ganglion (DRG) neurons, culture them on coverslips, and measure longest neurite outgrowth.

Keywords: Neurite outgrowth, Dorsal root ganglion (DRG), Glia, Laminin

Copyright: © 2016 The Authors; exclusive licensee Bio-protocol LLC.
How to cite:  Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
  1. Filous, A. R. and Silver, J. (2016). Neurite Outgrowth Assay. Bio-protocol 6(1): e1694. DOI: 10.21769/BioProtoc.1694.
  2. Filous, A. R., Tran, A., Howell, C. J., Busch, S. A., Evans, T. A., Stallcup, W. B., Kang, S. H., Bergles, D. E., Lee, S. I., Levine, J. M. and Silver, J. (2014). Entrapment via synaptic-like connections between NG2 proteoglycan+ cells and dystrophic axons in the lesion plays a role in regeneration failure after spinal cord injury. J Neurosci 34(49): 16369-16384.
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