Abstract
The gram-negative curved bacillus Vibrio cholerae (V. cholerae) causes the severe diarrheal illness cholera. The work presented here is to assess whether unsaturated fatty acids (UFAs), such as linoleic acid, have the potential to directly affect proteins involved in DNA binding because they are able to enter the cell. In this protocol, we show how to measure linoleic acid entering V. cholerae when added exogenously and determine whether it is able to enter the cytoplasm. This protocol will quantify how much linoleic acid is able to enter the cell and then identify the amount of linoleic acid that stays in the membrane or ultimately enters the cytoplasm.
Keywords: Vibrio cholerae, Cholera, Linoleic acid, Unsaturated fatty acids
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This work was supported by P. H. S. grants K22AI071011 and R56AI093622 and Bill and Melinda Gates Foundation grant OPP1068124 (to J. H. W.). This work is modified from previous work done in our laboratory (Thomson and Withey, 2014).
References
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