Abstract
The bone marrow is the site of hematopoiesis and contains mixed population of blood cells including erythrocytes, granulocytes, monocytes, dendritic cells, lymphocytes and hematopoietic stem cells. The following protocol provides a simple and fast method for isolation of bone marrow immune cells (no erythrocytes) from mouse femurs with a yield of approximate 8 x 107 cells in 5 ml culture media (1.6 x 104 cells/µl). Further isolation or flow cytometric analysis might be required for study of specific immune cell types.
Keywords: Blood, Flow cytometry, Hematopoietic stem cells
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was revised based on previous studies including the referenced articles below and was supported by an NIH grant (R21 MH099482) to Ning Quan.
References
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