Clonal Culture of Mouse Liver Progenitor Cells   

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Original research article

A brief version of this protocol appeared in:
Dec 2014


Liver stem/progenitor cells (LPCs) are defined as bipotential cells differentiating into both hepatocytes and cholangiocytes. For analyzing their differentiation potential, clonal culture has been used for LPCs isolated by a cell sorter. In addition, we can use the culture to assess functions of target genes on differentiation potential of LPCs. This protocol describes the process of cell isolation and colony assay to examine proliferative and differentiation potential of LPCs.

Keywords: Bipotential, Hepatocyte, Cholangiocyte, Liver, Flow cytometry

Copyright: © 2015 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Tanimizu, N. (2015). Clonal Culture of Mouse Liver Progenitor Cells. Bio-protocol 5(20): e1624. DOI: 10.21769/BioProtoc.1624.

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