Abstract
Purification and culture of endometrial epithelial cells (eEC) and stromal fibroblasts (eSF) from endometrial biopsies allows for downstream cell-specific in vitro studies. The utility of this protocol is the ease with which cells are purified without contamination from unwanted cell types, and the ability to use patient-paired eEC and eSF in experiments. These methods have been previously published, but here the protocol has been updated for maximum efficiency.
Keywords: Epithelial cells, Stromal fibroblasts, Human endometrium, Endometrial biopsy, Cell culture
Materials and Reagents
Equipment
Procedure
Troubleshooting
Recipes
Acknowledgments
We would like to acknowledge Dr. Linda C. Giudice, M.D., Ph.D., Dr. Juan C. Irwin, MD, Ph.D., and Kim Chi Vo. This work was funded in part by the F32HD074423 (JCC), the K99AI104262 (NRR), and a grant from the NIH, University of California, San Francisco-Gladstone Institute of Virology & Immunology Center for AIDS Research, P30-AI027763 (NRR).
References
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.