Abstract
Adult dorsal root ganglia neurons are among the few adult neuronal cell types that can be purified and grown relatively easily in dissociated cell culture. Here we describe a procedure for the isolation and growth of dissociated adult mouse DRG neurons using Percoll gradients and a chemically defined medium. These cultures can be used for morphological, biochemical and electrophysiological studies of neuronal growth and function.
Keywords: Primary culture, Dorsal root ganglia, Neuron, Percoll gradient, Rodent
Materials and Reagents
Equipment
Procedure
Acknowledgments
This work was supported by a grant from Spinal Cord Injury research board of New York State Department of Health.
References
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