Abstract
A wide range of stresses such as oxidative stress, acid, alkaline, UV, and metal can damage DNA. Here, we describe a protocol to measure the DNA nicking damage by Fenton reaction-mediated oxidative stress. Fenton reaction (Fe2+ + H2O2 → Fe3+ + OH- + ∙OH) produces the highly deleterious hydroxyl radicals that damage the cellular components such as DNA, lipid and proteins.
Keywords: DNA damage, DNA protection, Oxidative stress, Fenton reaction
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Acknowledgments
This protocol was modified from in vitro DNA damage assay described previously (Martinez and Kolter, 1997).
References
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