Abstract
Stereotaxic injection is an attractive approach for studying genetic, cellular and circuit functions in the brain. Injection of anatomical tracers, site-targeted lesions and gene delivery by recombinant adeno-associated viruses and lentiviruses in mice are powerful tools to study nervous system development and disease mechanisms. Stereotaxic injection of LPS or 6-hydroxydopamine has been used to establish animal models of Parkinson’s disease, the most common neurodegenerative movement disorder. Importantly, this protocol allows the manipulation of gene expression in the targeted rodent brain regions and even targeted cell types or a subpopulation of cells in the injected region at any postnatal developmental stage up to adulthood.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol has been developed and improved over the years by various researchers in Dr. Hong’s lab, especially Dr. Bin Liu (Gao et al., 2008).
References
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.
You may check out the following reference:Geiger B.M., Frank L.E., Caldera-Siu A.D., Pothos E.N. (2008). Survivable StereotaxicSurgery in Rodents . JoVE. 20. http://www.jove.com/index/Details.stp?ID=880, doi:10.3791/880Hope it helps.
I don't have such picture in hand. But online you can find a handful of them. Please check it out in the following two references: stereotaxic inj-1: from "Retrovirus-mediated single-cell gene knockout technique in adult newborn neurons in vivo". Tashiro,Zhao&Gage, Nature Protocols 1, 3049 - 3055 (2007).Stereotaxic inj-2: http://play.psych.mun.ca/~smilway/surgery-frame.html . The picture will show up by clicking "position the tip of the drill directly over Bregma."
I used the KDS310 (Nano Pump) from KD Scientific Inc., which is used exclusively with micro syringes.