Abstract
Here, we report a method for the transformation by electroporation of the human fungal pathogen Candida glabrata (C. glabrata). The protocol can be used for transformations in single well or in 96-well microtiter plates. It has been extensively used to generate a genome-scale gene deletion library using the C. glabrata background recipient strain ATCC2001 (Schwarzmüller et al., 2014).
Keywords: Candida glabrata, Transformation, High-Throughput, Gene deletion, Dominant selection
Materials and Reagents
Equipment
Procedure
Notes
Recipes
Acknowledgments
This protocol was adapted from a previously published method (Reuss et al., 2004). This work was supported by the Austrian Science Fund FWF through the ERA-Net Pathogenomics project FunPath (FWF-API-0125), and in part by grants from the Christian Doppler Society, the FP7 EC project FUNGITECT, the Marie-Curie ITN ImResFun (MC-ITN-606786) and the FWF Project FWF-P25333 „Chromatin“ to KK.
References
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