Abstract
Unbiased screening approaches are powerful tools enabling identification of novel players in biological processes. Chemical genetic screening refers to the technique of using a reporter response, such as expression of luciferase driven by a promoter of interest, to discover small molecules that affect a given process when applied to plants. These chemicals then act as tools for identification of regulatory components that could not otherwise be detected by forward genetic screens due to gene family redundancy or mutant lethality. This protocol describes a chemical genetic screen using Arabidopsis thaliana seedlings, which has led to recognition of novel players in the plant general stress response.
Materials and Reagents
Equipment
Software
Procedure
Notes
General screening notes
Recipes
Acknowledgments
This protocol is an expansion of that described in Bjornson et al. (2014). This work was supported by National Institute of Health (R01GM107311), and National Science Foundation (IOS-1036491and IOS-1352478), and Agricultural experimental station (CA-D-PLB-3510-H) grants awarded to KD.
References
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.