Abstract
The pollen tube, a fast tip-growing cell, is an excellent model to study membrane and cell wall biosynthesis. Here, we describe a simple protocol using an easy to use device to perform immunofluorescence labelling of pollen tube membrane and cell wall. The use of the NucleoSpin column to perform all the steps of the immunolabelling procedure results in obtaining more intact pollen tubes.
Keywords: Pollen tube, Cell wall, Membrane, Immunolabelling
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
Original versions of the protocols were described in Dardelle et al. (2010), Botté et al. (2011) and Dumont et al. (2014). This work was supported by the University of Rouen, the region Haute Normandie, and the “Trans Channel Wallnet” project that was selected by the INTERREG IVA program France (Channel) – England European cross-border cooperation program, which is co-financed by the European Regional Development Funds (ERDF). The work performed at LPCV (Grenoble) was supported by the Agence Nationale de la Recherche (ANR-10-BLAN-1524, ReGal; ANR BioAdapt Reglisse).
References
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