MIFE Technique-based Screening for Mesophyll K+ Retention for Crop Breeding for Salinity Tolerance   

Edited by
Tie Liu
Reviewed by
Anonymous reviewer
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In this protocol

Original research article

A brief version of this protocol appeared in:
Plant & Cell Physiology
Oct 2014


Potassium is known as a rate-limiting factor for crop yield and plays an important role in plants response under abiotic stresses. Recently, cytosolic K+ retention ability in leaf mesophyll has emerged as an important component of plant salt tolerance mechanism (Wu et al., 2013; Wu et al., 2014; Wu et al., 2015). In this protocol, the procedure for screening leaf mesophyll for K+ retention by the MIFE (microelectrode ion flux estimation) technique is described in detail using wheat as an example. By measuring NaCl-induced K+ efflux in leaf mesophyll, a large number of plant accessions can be screened and categorised according to their salinity stress tolerance. The method provides a rapid and reliable tool that targets the activity of specific membrane transporters directly contributing to salinity tolerance trait and, because of this, has a competitive advantage over traditional whole-plant phenotyping. While the focus of this protocol is on wheat, the suggested method may be adopted for screening K+ retention in leaf mesophyll in any other crop species.

Keywords: K+ retention, Ion flux, Salinity stress, Mesophyll cells, Breeding

Copyright: © 2015 The Authors; exclusive licensee Bio-protocol LLC.
How to cite: Wu, H., Shabala, L., Zhou, M. and Shabala, S. (2015). MIFE Technique-based Screening for Mesophyll K+ Retention for Crop Breeding for Salinity Tolerance. Bio-protocol 5(9): e1466. DOI: 10.21769/BioProtoc.1466.

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