Abstract
BioMag Goat Anti-Rat IgG is a standard BioMag particle coated with polyclonal goat anti-rat IgG antibodies and is highly suited for use in cell sorting methods where a rat IgG antibody is used as a primary antibody. BioMag Goat Anti-Rat IgG can be used to separate the cells of interest from a heterogeneous cell population using negative selection. BioMag Goat Anti-Rat IgG can also be used as a secondary antibody in enzyme immunoassays and radioassays that utilize a rat IgG primary monoclonal antibody.The efficiency of enrichment by negative selection depends on antigen availability and the total cell population. The author has intensive experience about enrichment of mouse CD4CD8 double negative (DN) thymocytes from total thymocytes which contains only about 2% DN population. Usually the DN population occupies more than 80% of total thymocyte after one round of enrichment. However the depletion efficiency is much lower for bone marrow cells.
Materials and Reagents
Equipment
Procedure
Acknowledgments
This protocol was adapted from Wang et al. (2009). Ying Zhao is thanked for technical assistance and members of the Sun laboratory are thanked for advice. This work was supported by the NIH.
References
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