Abstract
Here we provide the description of protocols to efficiently obtain protein extracts from embryos and ovaries of Drosophila melanogaster. These protocols are routinely applied in our laboratory and are based on two techniques: either embryos or ovaries are homogenized using a pestle and then the soluble proteins separated by centrifugation, or embryos are individually lysed by needle manipulation. The latter technique allows the use of small embryo numbers and the selection of specific developmental stages (Guilgur et al., 2014).
Keywords: Drosophila, Protein extracts, Embryos, Ovaires
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
We like to thank Paulo Navarro-Costa for critical reading of manuscript and Rui Martinho for his supervi-sion. Funding: FCT-Fundaçao para a Ciencia e Tecnologia (Portugal): Leonardo Gastón Guilgur, SFRH/BPD/47957/2008. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
References
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