Abstract
This protocol provides the possibility to isolate leukocytes including neutrophils out of intestinal tissues to use the received cells in further experiments of interest.
Materials and Reagents
Equipment
Procedure
Representative data
Several millions of leukocytes can be isolated out of the intestinal tract of a healthy mouse.
Notes
Fast working and cooling of the collected cells on ice optimizes the number of isolated leukocytes. Cells should be washed with PBS before proceeding to the experiments of interest to wash out the percoll.
Recipes
Acknowledgments
This work was supported by the DFG (ZE872/1-2 individual grant to RZ).
References
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