Abstract
Small RNAs like microRNAs (miRNAs), small interfering RNAs (siRNAs) and other noncoding RNAs including snRNA and snoRNA have tremendous impact on eukaryotic gene regulation. Extraction of high quality small RNAs is an important prerequisite for experimental analyses of miRNAs. This will prevent RNA degradation and remove associated contaminations including polyphenols, polysaccharides and other secondary metabolites. In this protocol we describe a simple way to isolate small RNAs from the leaf tissues of Vigna mungo combining the protocols of two commercially available kits with some modifications.
Keywords: MicroRNA, MYMIV, Next Gen Sequencing, Vigna mungo
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
The original version of this protocol was described in Paul et al. (2014). This work was supported by the Council of Scientific and Industrial Research, New‐Delhi, India for the Emeritus Scientist’s Project [Sanction No. 21 (0884)/12/EMR‐II].
References
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