Abstract
Hanging drop assay can be used to investigate cell-cell cohesion and cell-substratum adhesion through generation of 3D spheroids under physiological conditions. It also can be used to elucidate the role of cell-cell or cell-ECM interactions in specifying spatial relationships between two (or more) different cell populations. This simple method requires no specialized equipment and provides a means of generating tissue-like cellular aggregates for measurement of biomechanical properties for molecular and biochemical analysis in a physiologically relevant model.
Keywords: Hanging drop, Cancer, Aggregation, Migration
Materials and Reagents
Equipment
Procedure
Representative data
Figure 2. Photographs of representative spheroids from T-47D and MCF7 breast cancer cell lines. T-47D and MCF7 cells were cultured in hanging drops and formation of spheroids was observed at 2 and 4 days after culture.
Notes
Recipes
Acknowledgments
This study was supported, in part, by the National Institutes of Health Grant CA120510.
References
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