Abstract
Variety of immunological and biochemical studies associated with infection or inflammation in the central nervous system (CNS) utilize CNS-resident and/or infiltrating cells which were isolated from the CNS of naïve and affected mice in order to investigate the underlying mechanisms and the potential roles of the cell populations. Mechanical and enzyme-based single cell preparations of CNS cells are subjected to a density gradient to obtain functional single cells. In combination with cell-specific biomarkers, the function and/or status of resident microglia and infiltrating lymphocytes, including B and T cells as well as macrophages, can be characterized.
Materials and Reagents
Equipment
Procedure
Representative data
Figure 2. A representative result from continuous Percoll gradient of TMEV-infected CNS cells Flow cytometric analysis of CNS infiltrating cells isolated using continuous Percoll gradient at 7 days post infection is shown.
Notes
Recipes
Acknowledgments
This protocol was adopted from many previous studies including the referenced work below and was supported by NIH (2RO1 NS28752 and NS33008) and NMSS (RG 4001A6 and RG 4342A7) grants.
References
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