Abstract
Drug-induced mitochondrial injury can be caused by many different mechanisms including inhibition of mitochondrial DNA replication, transcription, translation, and altered protein function. Determination of the level of mitochondrial protein synthesis, or mitochondrial biogenesis, relative to the cellular protein synthesis, provides important information on potential mitochondrial toxicity.
Keywords: Mitochondrial toxicity, Mitochondrial protein synthesis, Nucleoside-related toxicity, Chloramphenicol, MitoBiogenesis™ In-Cell ELISA Kit
Materials and Reagents
Equipment
Software
Procedure
Notes
Recipes
Acknowledgments
All of the work was sponsored by Gilead Sciences, Inc. This protocol was adapted from Feng et al. (2014).
References
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