Abstract
Botrytis cinerea (B. cinerea) is an aggressive fungal pathogen that infects more than 200 plant species. Furthermore, the pathogen can attack fruits of some plants, such as tomato and apple. B. cinerea has become one of the model systems in molecular phytopathology because of its economic importance and sophisticated genetic operation methods. Virulence assays are very important in the study of fungal pathogenesis. This protocol details the artificial inoculation procedure of B. cinerea on tomato and apple fruits. It also can be used to analyse the virulence of postharvest fungal pathogens on other fruits, such as pear, peach, jujube and so on.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was adapted from Zhu et al. (2010) and Chan et al. (2007). The work was supported by the 973 program (grant number 2013CB127103), by National Natural Science Foundation of China (grant number 31030051) and by the Chinese Academy of Sciences (grant number KSCX2-EW-G-6).
References
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