Abstract
Latency and reactivation of human cytomegalovirus (HCMV) is intimately associated with the myeloid lineage. Multiple studies have used in vitro protocols to generate dendritic cells (DCs) from myeloid precursors. Here we describe the direct isolation of DCs from peripheral blood to study HCMV latency directly in this cell type.
Keywords: Dendritic cell, Cell purification, Cytomegalovirus
Materials and Reagents
Equipment
Procedure
Representative data
Figure 1. Isolation of PBMC on histopaque 1077
Figure 2. FACs analysis of putative DCs post isolation shown to be HLA-DR positive and CD14, 19 and TCRα/β negative
Recipes
References
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.