Abstract
This protocol describes the construction of Caco-2 stable cell lines using the Lipofectamine transfection method.
Materials and Reagents
Equipment
Procedure
Acknowledgments
This protocol was adapted from Reference 1 in the Eric Sibley lab, Department of Pediatrics, Stanford University, CA, USA. Funding from the NIH supported this work.
References
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E is 5.1ml DMEM with 2x FBS. (Here 2 x FBS refers to the double amount of whatever working concentration of FBS you use for regular cell culturing.). I hope I answered your question.