Published: Vol 4, Iss 19, Oct 5, 2014 DOI: 10.21769/BioProtoc.1255 Views: 14626
Reviewed by: Oneil G. BhalalaXuecai Ge
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Abstract
This protocol is useful to manipulate gene expression in the embryonic retina and compare the result with the contralateral non electroporated retina. In addition, the electroporation of a membrane or cytoplasmic tagged GFP allows to determine the effects of gene manipulation on the outgrowth of retinal ganglion cell axons (Garcia-Frigola et al., 2007) or simply to follow axon outgrowth in mutant embryos. DNA can be directed to different quadrants of the retina (ventral or dorsally) by modifying the position of the electrodes (Petros et al., 2009; Sánchez-Arrones et al., 2013). After the procedure, embryos are left developing to the desired stage, including postnatal stages.
Keywords: Gene ExpressionMaterials and Reagents
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Procedure
Representative data
Recipes
Acknowledgments
We would like to thank Paola Bovolenta for her support and critical reading. We are grateful to Eloisa Herrera and Cristina Garcia-Frigola for the adapted protocol.
References
Article Information
Copyright
© 2014 The Authors; exclusive licensee Bio-protocol LLC.
How to cite
Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
Category
Neuroscience > Development > Neuron
Neuroscience > Neuroanatomy and circuitry > Animal model
Developmental Biology > Morphogenesis
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