Abstract
It is becoming increasingly apparent that stress signalling is important for tolerance of fungal species to antifungal chemicals and proteins. The high-osmolarity glycerol (HOG) pathway responds to a number of stressors including osmotic and oxidative stress. This protocol describes a method to detect activation of the Candida albicans (C. albicans) MAPK Hog1 by monitoring its phosphorylation in response to an antifungal protein.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol has been adapted from Hayes et al. (2013), in which a brief description of the protocol was presented.
References
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