Abstract
We developed an in vivo method to assay plant transcription factor (TF)–promoter interactions using the transient expression system in Nicotiana benthamiana (N. benthamiana) plants. The system uses the Arabidopsis stay green (SGR) gene as a reporter. Induction of SGR expression in N. benthamiana causes chlorophyll degradation and causes leaves to turn yellow.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol is adapted from the following paper: Ma et al. (2013). This work is supported by National Science Foundation grants DBI-0723722 and DBI-1042344, and UC Davis funds to SPDK.
References
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