Abstract
An Arabidopsis leaf blade is composed of many layers that are sandwiched between two layers of tough skin cells (called the epidermis). Four layers (adaxial epidermis, palisade layer, spongy mesophyll and abaxial epidermis) contain specialized cells. Here we describe a quick and simple method for analyzing the size, number and density of different types of cells in an Arabidopsis leaf blade. This method would be of interest to people who would like to investigate cell size and number changs in different cell layers in leaves or leaf-like organs without having to dissect the samples.
Materials and Reagents
Equipment
Software
Procedure
Recipes
Acknowledgments
This protocol was developed in association with the study as previously published (Cheng et al., 2013). It was expanded and the full protocol with additional data is only described here. Y.Z. thanks the Ministry of Science and Technology and National Natural Science Foundation of China, and H.W. thanks the Natural Sciences and Engineering Research Council of Canada for financial support.
References
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It should. You just have to capture the image using the software that controls your microscope and save it as a TIFF file so that you can import it into ImageJ to use the plug-in.