Abstract
Most recently a novel avian-origin influenza A (H7N9) virus emerged in China and has been associated with lots of human infection and fatal cases. Molecular diagnostic methods are thus urgently needed in public health laboratories. We developed a SYBR green-based one-step real time reverse transcription-PCR (RT-PCR) to detect the novel H7N9 virus.
Materials and Reagents
Equipment
Software
Procedure
Acknowledgments
This experimental protocol was partly adapted from the previously published paper: Zhu et al. (2013). This protocol was supported in part by the Jiangsu Province Health Development Project with Science and Education (ZX201109), the Jiangsu Province Key Medical Talent Foundation (JKRC2011002, RC2011191), and the Science and Technology Pillar program of Jiangsu Province (BE2011796). We thank Dr. Shu Yuelong, China National Influenza Center, Chinese Center for Disease Control and Prevention, Beijing, China, for providing the entire gene sequence data of the first three H7N9 virus isolates used for primer design.
References
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