Abstract
Macrophage depletion has been used extensively to study autoimmune disease and more recently in tumor models. The clodronate-containing liposomes will be recognized as foreign particles and get engulfed by macrophages upon dosing into the animal by the chosen routes. Consequently, macrophages that have engulfed liposomes will all be destroyed by the liposomal. In the protocol presented here the clodronate-containing liposomes were used to systemically deplete macrophages in mice.
Materials and Reagents
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Acknowledgments
We thank Yinling Hu, Robert H. Wiltrout and Jami Willette-Brown from Frederick National Laboratory for Cancer Research for their support and suggestions on the experimental design. This work was supported by the National Cancer Institute (ZIA BC 011212 and ZIA BC 011391 to Y.H.).
References
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